Antibody Development

It all begins with discovery.

TrueBinding would not be where it is now, in an advanced phase of Stage II clinical trials for Alzheimer’s disease, without its full-fledged, high-throughput platform of cloning, assay development, and sequencing, now available to help you achieve your antibody discovery and preclinical characterization goals ever faster.

We offer a rare (unique) abundance of antibody discovery and development services to organizations in academia and industries of all stages and backgrounds. Get access to multiple technology platforms with optimized workflows for immunizations, polyclonal antibody preparation screening, monoclonal antibody isolation, sequencing, antibody genes synthesis, recombinant antibody expression, characterization, antibody humanization, all tailored to your needs and more.

Launch farther with TrueBinding.

Services

Hybridoma Technology

Your Trusted Expert in Hybridoma Technology for Monoclonal Antibody Development

TrueBinding is pleased to offer our complete hybridoma workflow to scientists worldwide. We produce and characterize high-quality monoclonal antibodies (mAbs) with an advanced customisable platform that seamlessly transitions from antibody discovery to downstream production. Our utilization of ELISA and FACS-based high-throughput screening enables rapid analyses of diverse antibody candidates for specificity, cross-reactivity, and functionality.

Immunization: We tailor our comprehensive immunization strategies to meet a variety of project needs, including DNA immunization, whole-cell immunization, and other approaches. Our goal is to elicit a robust immune response that leads to the generation of high-quality antibodies.

Hybridoma Generation and Screening: TrueBinding employs optimized fusion protocols that enables efficient electrofusion and PEG fusion of splenocytes with suitable myeloma cells for successful hybridoma formation. We then screen for hybridomas with high specificity to the target antigen.

Antibody Expression and Purification: Our stringent purification process is capable of producing high-quality, pure monoclonal antibodies up to gram-level quantities with low endotoxin levels (≤ 1 EU/mg).

Single B-cell Technology

TrueBinding offers an efficient single B-cell sorting platform that ensures the generation of diverse and functional monoclonal antibodies. We preserve the critical VH-VL pairing as found in native B-cells, maintaining the antibody's original characteristics and functionality. Our comprehensive workflow includes single B-cell isolation, screening, sequencing, and cloning which guarantees the generation of high-affinity mAbs in a rapid and precise manner.

Our Single B-cell Antibody Discovery Workflow:

Isolation of Single B-cells:

We use thorough and optimized sorting methods to isolate single B-cells from immunized mice in an antigen-selective manner. FACS in particular allows for choice in the specific mAbs recovered.

Single B-cell Antibody Sequencing and expression:

Through single-cell cDNA synthesis, we amplify variable antibody gene transcripts. We then use plasmid-free systems (i.e. linear cassettes) for efficient expression of mAbs.

Screening of Antibodies:

We express, purify, and test the reactivity and biophysical characteristics of produced mAbs to select those that are suitable for your specific research or therapeutic needs.

Antibody Display Library Services

Give your project the strong start it needs

TrueBinding creates display antibody libraries and antibody optimization platforms with therapeutic developability in mind, leading to robust and rapid antibody discovery and enabling you to advance your projects with confidence.

We feature multiple comprehensive, customizable library construction services under a variety of phage & mammalian display systems:

Naïve Antibody Library service

Immune Antibody Library service

Sequencing

Antibody Engineering

Humanization

Leveraging our substantial expertise in antibody humanization which has yielded favorable outcomes in Phase I clinical trials, TrueBinding offers a proven process to produce top-tier humanized antibodies.

We incorporate CDR grafting, chain shuffling, humanized IgG library screening and more in our comprehensive workflow to ensure the production of humanized antibodies with the reactivity characteristics of the parent antibody conserved.

Antibody Affinity maturation

Enhance your antibodies beyond their natural biophysical limitations with TrueBinding’s affinity maturation system using synthetic antibody libraries. Our approach involves controlled in vitro selections for the rapid isolation of antibodies with even higher affinity and specificity. The resulting antibodies are ideal candidates for therapeutic and diagnostic applications.

Epitope Mapping analysis

Epitope mapping plays a crucial role in understanding the interaction between proteins and their corresponding antigens, making it an essential step in developing potent drugs and protecting patented products. TrueBinding is at the forefront of epitope mapping analysis and has achieved a remarkable success rate of over 95% for several monoclonal antibodies (mAbs).

 ●      Hydrogen/deuterium (H/D) exchange

 ●      Peptide Scanning

 ●      Alanine Scanning

Molecular Biology Services

TrueBinding specializes in handling the following Molecular Biology applications on your behalf, allowing you to direct your focus towards research. We deliver top-notch quality products at rapid turnaround times so that your research operations proceed smoothly.

Vector Cloning

●      In cloning custom vectors, we will work with you to determine your needs and customize the project accordingly. Multiple methodologies are available to create the desired construct as quickly and affordable as possible.

Plasmid Preparation

Swiftly producing top-tier plasmid DNA in any desired quantity from diverse source materials is within our realm of expertise at TrueBinding. Our services encompass sequence verification, including primer walk if deemed necessary, alongside rigorous quality checks through restriction digestion for each preparation.

Site-directed mutagenesis and truncations

All that is needed is a vector map and nucleotide sequence of the insert, and TrueBinding can handle the rest. We incorporate all sorts of plasmid modifications including but not limited to point mutations, truncations, and insertions to deliver the custom plasmid you are looking for.

Antibody reformatting and high throughput cloning

TrueBinding proudly presents our robust and high-throughput cloning protocol for efficient antibody reformatting. Try out our cutting-edge approach which ensures the incorporation of a resilient leader sequence and offers the flexibility to select human or mouse isotypes.

Protein Engineering and Purification

Our goal at TrueBinding is to provide a one-stop solution to any protein expression venture. We have an extensive record of success in effectively managing and producing a diverse range of intricate recombinant proteins. Whether your project necessitates meticulous small-scale research or the scale and precision of large-scale cGMP production, your final recombinant antibody products will be delivered to support your scientific research in a timely and cost-effective manner.

We offer a comprehensive suite of services such as E. coli expression, mammalian expression, and meticulous antigen preparation, as well as various means of protein purification by affinity and chromatography.

To obtain a personalized quotation or to articulate your specific project needs, we cordially invite you to contact us. It will be our pleasure to provide our expertise and contribute to the success of your project.